Nitrofuran (AMOZ) ELISA Test Kit
Catalog No. LSY-10001
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of AMOZ in the sample. The coupling antigens are pre-coated on the micro-well stripes. The AMOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AMOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AMOZ in it. This value is compared to the standard curve and the AMOZ concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.05 ppb
Incubation Temperature: 25℃
Incubation Time: 60min～15min
Detection limit
Tissue, liver 0.1 ppb
Honey, milk, intestine 0.1 ppb
Fish, shrimp (some interference) 0.15 ppb
Cross-reaction rate
AMOZ 100%
AHD <0.1%
AOZ <0.1%
SEM <0.1%
Recovery rate
Tissue, liver 80±25%
Honey, milk, intestine 75±17%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb
3） Enzyme conjugate (7 mL) red cap
4） Antibody working solution (7 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 2× concentrated redissolving solution (50 mL) transparent cap
10） 2-Nitrobenzaldehyde (C7H5NO3)(10 mL) white cap